The mission of the Vector and Genome Engineering Facility (VGEF), an innovative academic scientific core facility at Children’s Medical Research Institute (CMRI), is to support basic and translational research in vector-based gene editing and therapy to target genetic diseases.
VGEF first opened its doors in 2016, and now occupies an entire floor of the newly constructed CMRI Tower. Our facilities include three tissue culture lab spaces, and are custom designed to accommodate work relating to vectorology and CRISPR gene editing in both immortalised and primary cells.
The two main branches of VGEF are: Vectorology, which focuses on the packaging of lentiviral, adenoviral and AAV vectors; and Genome Engineering, which carries out customised gene editing projects. In addition to being a service facility that provides investigators with access to the latest CRISPR technology and viral-vectors, including many novel AAV serotypes, VGEF is also active in developing new and improving existing technologies that will empower its customers and the wider scientific community.
In alignment with our goal to promote vector-based and gene editing technologies, VGEF offers consultation in study design and technical support in the selection of appropriate tools for specific experimental needs. Beyond our academic and commercial services, we also engage in collaborative studies that are aligned with our research focus in AAV biology, vector development and gene editing.
Facebook: @CMRI.VGEF.TVG (https://www.facebook.com/CMRI.VGEF.TVG)
If you want to request a reagent, want to ask about specific unpublished results or want to initiate collaboration, please do not use our social site but send us an email.
VGEF will consider special requests from academic and commercial customers for consulting and/or hands-on training in vector production and genome editing. All interested parties should email VGEF at [email protected].
If you have relevant skills and are looking for a job, or want to get out of your dead-end and boring job, check out VGEF!
We are looking for people who:
AAV CsCl PEI Production
AAV CsCl PEI Transfection Template
AAV Iodixanol PEI Production
AAV Iodixanol PEI transfection template
AAV qPCR titration protocol
Adenoviral vector production
Ad ELISA Template file
Ad ELISA Titering
Ad ELISA VR-1516
Ad qPCR Titration protocol
Clontech Rapid Ad titration 632250
Kimberley Dilworth, BS, Research Assistant
Grober Baltazar Torres, BS, Research Assistant
Matthieu Drouyer, PhD, Research Officer
Arek Rybicki, MS, Research Assistant
Martin Pagac, PhD, Research Officer
Boaz Ng, Research Assistant